Two egg samples from each treatment group were
collected during the 101st wk of age from the previous
24 h of production. From the first set, 12 eggs from
each replicate were broken into a stomacher bag and
then stomached for 60 s in accordance with the procedures
of Anderson (2011). The whole egg samples
were divided into six 50-mL conical tubes and frozen at
−29°C. Each of the samples were divided into 3 identical sets of 2 to 50 mL conical tubes of whole egg, then
shipped on dry ice to 3 different commercial testing
laboratories with a minimum of 2 conical tubes of −50-
mL samples sent to each laboratory