Reaction mixtures (25 μl) contained 1.6 μM each of CMgBFIP and CMgBBIP, 0.2 μM each of outer primer (CMgBF3 and CMgBB3), 0.8 μM each of loop primer (CMgBLPF and CMgBLPB), 2× reaction mixture (12.5 μl), Bst DNA polymerase (1 μl), and 5 μl of target DNA.