In this work, we have developed a sandwich electrochemical immunosensor where PB@HAP was used as carriers of HRP and antibody for the analysis of AFP, an important tumor marker useful for hepatocellular carcinoma. We proved in this work the concept that PB@HAP and GS could be used successfully to enhance the performances of classical sandwich immunosensor, achieving higher sensitivities. Additionally, PB@HAP can be easily synthesized and functionalized, therefore, represents an interesting characteristic to improve sandwich electrochemical immunosensor. Furthermore, the screen-printed electrodes can be used instead of the GCE in order to achieve mass production and it has potential application in the detection of other tumor markers