Micrographical observations revealed epiphytic bacterial
interaction with the host plant through surface
colonization, in which the microbes were seen adhered to
the external plant tissue as individual cells or clusters. SEM
recovered higher density of epiphytic bacteria from the
ventral surface of the leaf (Fig. 2) compared to the dorsal
surface which corroborates to reports of Lalke-Porczyk and
Donderski [21]. This may be due to comparatively lower
environmental stress such as, reduced radiation, rainfall
and higher stomatal openings on the ventral side of the
leaves. Homogeneous as well as heterogeneous populations
were observed using SEM among the phyllobacteria
and rhizobacteria of ethnomedicinal plants (Fig. 3). Microscopic
technique revealed the patchy recovery of bacteria
from leaves in leaf imprint studies which suggests thatbacteria do not occur in a uniform pattern across leaf surfaces
but tend to be localized in particular sites [22–24].
The most frequent sites of bacterial colonization were the
stomata (Fig. 2), epidermal cell grooves along the veins
(Fig. 3), at the base of trichomes and root hairs [25,26].
These observations suggest a relatively scattered colonization
and variation in population sizes, embedded in an
exopolymeric matrix as reported on leaf surfaces of different
plant species [27].
Micrographical observations revealed epiphytic bacterialinteraction with the host plant through surfacecolonization, in which the microbes were seen adhered tothe external plant tissue as individual cells or clusters. SEMrecovered higher density of epiphytic bacteria from theventral surface of the leaf (Fig. 2) compared to the dorsalsurface which corroborates to reports of Lalke-Porczyk andDonderski [21]. This may be due to comparatively lowerenvironmental stress such as, reduced radiation, rainfalland higher stomatal openings on the ventral side of theleaves. Homogeneous as well as heterogeneous populationswere observed using SEM among the phyllobacteriaand rhizobacteria of ethnomedicinal plants (Fig. 3). Microscopictechnique revealed the patchy recovery of bacteriafrom leaves in leaf imprint studies which suggests thatbacteria do not occur in a uniform pattern across leaf surfacesbut tend to be localized in particular sites [22–24].The most frequent sites of bacterial colonization were thestomata (Fig. 2), epidermal cell grooves along the veins(Fig. 3), at the base of trichomes and root hairs [25,26].These observations suggest a relatively scattered colonizationand variation in population sizes, embedded in anexopolymeric matrix as reported on leaf surfaces of differentplant species [27].
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