The hydrolysis of urea is a useful method to
help identify several genera of yeastlike organisms
including Cryptococcus, Rhodotorula,
Trichosporon, and occasionally Candida krusei.
In a previous report (3), a rapid method for
demonstrating urease production was described
that proved helpful for the early detection of
cryptococci recovered from clinical specimens.
When used with other rapid presumptive tests
(1, 2), the rapid urease test provides a tentative
identification of Cryptococcus neoformans
within 6 h after initial recovery.
The following report describes a simplified,
more rapid system, similar in method to the
rapid nitrate utilization test (2), that selectively
detected the urease activity of 99.6% of 286
isolates of C. neoformans within 15 min.
The hydrolysis of urea is a useful method tohelp identify several genera of yeastlike organismsincluding Cryptococcus, Rhodotorula,Trichosporon, and occasionally Candida krusei.In a previous report (3), a rapid method fordemonstrating urease production was describedthat proved helpful for the early detection ofcryptococci recovered from clinical specimens.When used with other rapid presumptive tests(1, 2), the rapid urease test provides a tentativeidentification of Cryptococcus neoformanswithin 6 h after initial recovery.The following report describes a simplified,more rapid system, similar in method to therapid nitrate utilization test (2), that selectivelydetected the urease activity of 99.6% of 286isolates of C. neoformans within 15 min.
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