Interestingly, damage was seen in stage IV oocytes
following a longer exposure period. Following 72 h of
exposure, stage IV oocytes demonstrated expulsion of
cytoplasmic contents. Rupturing of the follicular wall
caused cytoplasmic contents to spill out, oftentimes
resulting in a loss of the nuclear material. Additionally,
anucleated stage IV oocytes became more apparent as
exposure time increased. Such damage to these follicles,
in turn, would have an effect on those follicles’
production of estradiol.