shoot to total Zn content with increasing N supply suggests that optimising N supply promotes the root-to-shoot Zn translocation and results in greater overall shoot Zn accumulation. Shoot Zn accumulation positively correlated with grain yield and grain Zn concentration. This relationship indicates that higher shoot Zn accumulation, resulting from greater N supply, may maximize grain yield and grain Zn concentration. The positive effects of N on root Zn uptake, root-to-shoot Zn translocation, and Zn accumulation in grain and shoot are of significance for Zn biofortification strategies in cereals and improving grain yields.