Preculture of each species was performed under continuous conditions in a CO2 incubator (GXZ-300D;
Jiangnan Instrument Factory, Ningbo, China) illuminated using fluorescent tubes at 110μmol photons m−2
s−1 irradiance for a 12:12-h light: dark cycle at 20 (±1) °C for two days, and continuous aeration was provided using current ambient air.