2.3.3 Alcohol plant extract
Following the method described by Khan et al. (2004), fresh
leaves of the selected indigenous plants were collected and airdried.
The dried leaves were ground in a grinder to a powder
state, subjected to cold extraction with 95 per cent alcohol for 8
days, and then filtered through two layers of cheesecloth.
2.3.4 Effect of botanicals on mycelial growth of anthracnose
fungus
The effect of the plant extracts on the linear mycelial growth was
evaluated using hole-plate diffusion method of [8]. Petri dishes
containing 15 milliliters of potato dextrose agar each were
inoculated with 5-millimeter disc of fungal pathogen at the center
surrounded by 3 wells of 1 centimeter each in diameter at a
distance of 1 centimeter from the fungal pathogen. Each well was,
added with 100-micron liter of the aqueous or ethanol extracted
plant extracts. Three plates were used for each plant extract as
replicates and sterile water was used for control. Inoculated
plates were incubated at 25 to 30oCelsius until mycelial growth of
fungal pathogen covered the surface of the agar medium in
control treatment. The antifungal activity of each plant extract was
measured by measuring the mycelial growth of the pathogen on
PDA with measuring rule and the percentage of linear growth
reduction (LGR) of fungal pathogen in relation to the control was,
calculated using the following formula: