Experimental design and feeding
Experimental design: the cattle and
buffaloes were fed of four experimental diets of
KG, EG, EM and RS according to a Latin Square
(4x4) design for each type of animal. Each period
consisted of 10 days adaptation followed by 4 days
collection of faeces, urine and rumen fl uids. The
animals were housed individually in metabolism
cages. Daily feed allowance was restricted to 70 g
dry matter per kg M0·75 to ensure that all food was
eaten. The animals were fed daily at 8:00, 14:00
and 20:00 h. Feed refusals were daily recorded (if
any remain); water was always available.