In order to optimize shoot elongation, stem segments (2.0 cm)
containing two nodes were excised from plants obtained 60 days
after initiation of primary cultures, and inoculated on B5, ½ B5
or ½ MSM medium supplemented with 5 or 10% coconut water
(Sigma–AldrichChemicalCo.). Threeflasks containing four explants
each were cultured per treatment and maintained under standard
culture conditions for 30 days. Experiments were repeated twice.