The final filtration led to represent the balsam (tincture) of propolis and is referred to as EEP (ethanolic extract of propolis). The solutions were evaporated to near dryness on a rotary evaporator (EYELA Rotary Evaporator N-100) under reduced pressure at 40°C. The remaining extract was incubated at 37°C for two weeks till the remainder of the ethanol was evaporated and the resulting powders were kept at -20°C. For experimental treatments, EEP at 100 mg/ml concentration was dissolved in DMSO as solvent and stored at -20°C. Cell line and culture AGS human gastric cancer cell-line was purchased