Single cell suspensions were prepared from 0.02% EDTA- treated monolayers which were washed and diluted in phosphate-buffered saline (PBS) prior to counting and inoculation. Cells were storedice until injection.
Single cell suspensions were prepared from 0.02% EDTA- treated monolayers which were washed and diluted in phosphate-buffered saline (PBS) prior to counting and inoculation. Cells were storedice until injection.