In the present study, two geographically different locations were studied. Geographically, northern and southern regions of India are distinct in terms of environmental conditions. M. restricta and M. globosa were the two most prevalent species isolated from dandruff patients of northern India having moderate to severe lesions while M. furfur, M. restricta and M. globosa were either isolated singly or in mixture from patients from southern India. In Japan and USA, M. globosa is most frequently isolated from dandruff cases followed by M. restricta13. Whereas M. furfur and M. slooffiae are isolated from scalp of healthy individuals and are considered as commensal species. In the present study, the sample collection