Lyophilized chicken pancreas conjugase (Pel-Freez Biologicals,
Rogers AR, USA) solution (5 mg/ml) was prepared according to
Shrestha et al., with minor modifications [12]. Chicken pancreas
powder was dispersed in 0.1 M sodium phosphate buffer with
2% sodium ascorbate (pH 6.0) centrifuged at room temperature
and the supernatant collected for use for folate deconjugation in
wine.