In the present study, we described changes in turkey semen motility parameters and proacrosin/acrosin sys- tem during liquid storage and after cryopreservation. Anti-acrosin I antibodies were produced and used to
demonstrate Western blot analysis profiles of sperm extract. These profiles were different regarding pres- ence or absence of inhibitors (NPGB and Kazal family inhibitor) during the extraction process. During liquid storage a decrease in sperm motility and an increase in sperm-extracted amidase activity were observed. How- ever, no changes in Western blot analysis profiles of sperm extract and seminal plasma were visible during liquid storage. After cryopreservation a decrease in sperm motility and all sperm motility parameters were observed. In contrast to liquid storage, cryopreservation did not increase extracted amidase activity. However, changes in Western blot analysis profiles were visible in sperm extract and seminal plasma after cryopreser- vation. Extracted amidase activity did not correlate