One milliliter of pre-inoculated BPW were transferred
to 10 mL of selective enrichment media rappaport
vassiliadis soyabean meal broth (RVSM) (BioMark, India)
and tetrathionate broth (TTB) (BioMark) and incubated
further at 37 °C for 24 h. On the following day, two loopful
of RVSM and TTB were transferred to bismuth sulfite agar