Material and methods
Plant material
Stem cuttings and leaf samples from sugarcane plants were collected in 2010 in the experimental field of the Khon Kaen Field Crop Research Center at Thapra experimental site (Khon Kaen, Isaan), at the Cane and Sugar Industry Promotion Center research station (Bang Phra, Chonburi province), in farmer fields nearby Chonburi and from plants at Kanchanaburi plantation (in Kanchanaburi province). From each plant 2–3 leaf samples were collected. The leaf samples were sun-dried and brought to Bayreuth for analysis.
DNA extraction