Somatic embryogenesis was induced according to García
and Menéndez (1987). For callus induction, leaf sections
of coffee (Coffea arabica cv. 'Catimor') were cultured for
four months in medium containing half strength
Murashige and Skoog (1962) salts, 30 g/l sucrose, 10 mg/l
thiamine, 100 mg/l myo-inositol, 35 mg/l cysteine, 1 mg/l
2,4-dichlorophenoxyacetic acid, 8 mg/l 6-bencyladenine
and 8 g/l agar. The resulting calli were transferred to a
medium of similar composition but with 0.8 mg/l
naphthalene acetic acid as a sole plant growth regulator.