With rapidly developing industries, fossil fuel is greatlyemployed as fuel for energy source, which results in greenhouse effect and environmental destruction. Therefore,developing sustainable energy is important to our ecological environment. When it comes to fermentative biohydrogen production, it acquires with many advantages such as organic degradation with low cost, high heat and clean energy output,
operation without light and low pollution. Several researchersfocused on two-stage of biohydrogen and biomethane production technology for enhancing energy recovery efficiency. According to previously research studies, some of researchers used one-stage and two-stage system to compare the potential of commercial feasibility. The result has shown that thetwo-stage system has advantage to enhance bioenergy production