Hairy Roots Elicitation with MeJA
MeJA solutions were prepared by diluting the MeJA stock
(Aldrich, 95 ? % purity) in absolute ethanol (J. T. Baker,
99.8%). Different concentrations of MeJA (10, 100 and
250 lM) were added to 15-day-old hairy root culture and
for the control treatment absolute ethanol alone was used.
Root tissue and culture media were collected at 12, 24, 48
and 72 h after elicitation.