2.2. Preparing partial decayed specimens
The method of making specimens which was decayed partially by brown rot fungi was proposed, as
shown in table 1. The specimens were continuously attacked by brown rot fungi for 7 weeks. Figure 2
shows the state of the specimen.
Table 1: Method of preparing specimens which is deteriorated partially by brown rot fungi
Contents
1
Autoclave sterilize the vermiculite culture containing liquid culture (B culture*), the nonwoven fabric bag, and wet paper
towel (wetting mat).
2 Inoculate brown rot fungi Fomitopsis palustris on the sterilized vermiculite culture.
3 Pack 6 g of inoculated vermiculite culture into the sterilized nonwoven fabric bag (fungal mat).
4 Brush all surface of specimen with Alcohol.
5
Put the fungal mat on the nailed face of specimen, and the wetting mat on the side faces.
Wrap around the mats with PVC film to keep wet condition (Figure 2).
6
Cultivate the wrapped specimens at 27 degrees, RH83% for 7 weeks, supplying with 10ml water to each mat every two
weeks.