The sterilized nodal explants were inoculated vertically on the medium supplemented with different concentrations of 6- benzylaminopurine (BAP) and kinetin (ranging from 1.0–6.0 mg/L)
alone or in combination with IAA (0.1–1.0 mg/L). The regenerated shoots from the nodal meristems of explants were further multiplied on MS semi-solid (with agar) as well as in liquid media
(without agar) in 250 mL culture flask. The media were fortified withBAP and kinetin(0.1–3.0 mg/L) and IAA(0.1–1.0 mg/L)formultiplication of shoots in cultures. Each flask bearing one regenerated shoot allowed for 5 weeks of incubation in growth room. The number
and length of the shoots were measured after 3rd and 4th cycle of shoots multiplication