The phenol concentration of the samples was determined by colorimetric method [17]. Flasks with 50 ml of Sabouraud broth (Difco Laboratories, Detroit, MI) with chloramphenicol (0.05% w/v) were inoculated with 5 ml of each sample, and incubated for 48 h, at 28?C, under shaking conditions (160 rev min?1). After this period several slides were prepared to evaluate the isolated biodiversity and to verify the presence of yeasts, the main object of this work. Additionally, aliquots of 100 ll of each culture were plated on Sabouraud agar (Difco Laboratories, Detroit, MI) with chloramphenicol (0.05% w/v) for isolation of colonies. The Petri dishes were incubated at 28?C and examined daily for a week.