Multiparous Holstein cows, 32, were blocked according to milk yield divided into four
treatments being: C) Control group, cows cooled before milking time (0500 and 1700 h daily);
AM) cows cooled at 1100 h and before milking; PM) cows cooled at 2300 h and before milking;
and AM+PM) cows cooled at 1100 and 2300 h, as well as before milking. Total cooling time
per group was 1 h for the Control group, 2 h for AM and PM groups, and 3 h for the AM+PM
group. Cows were moved to a holding pen daily to be cooled.