Figure 4. Western blots prepared from 18% gels of (a) nonstimulated (NS) and (b) electrically stimulated (ES)
samples were labeled with TN-T monoclonal antibody. The TN-T band that comigrates with the purified TN-T
standard (not shown) and the other two TN-T isoforms (one above, one below) decrease as postmortem (PM) time
increases and are light bands at 28 d. A 30-kDa polypeptide is somewhat more heavily labeled from 7 d to 28 d in ES
samples.