Agar plate containing Rhodamine B 0.001 % (w/v),
nutrient broth 0.8 %, NaCl 0.4 % (w/v), agar 1 % (w/v) and
olive oil 2 % was prepared in distilled water, adjusted pH of
6.5. The assay incubated at 55 C for 18 h and lypolitic
activity was identified as an orange halo around colonies
under UV light at 350 nm.