Soxhlet extraction
2 g of dried E. arvense herb powder was placed to the filter paper,
and extracted with applicable solvent (methanol or 80% aqueous
solution of methanol) for 48 h in Soxhlet apparatus. Extracts were
evaporated to dryness. The residues were dissolved in 25 mL of 80%
aqueous solution of methanol. The whole procedure was repeated
three times for each solvent.
Ultrasound assisted extraction (USAE)
2 g of dried E. arvense herb powder was extracted with 50 mL of
applicable solvent (methanol or 80% aqueous solution of methanol)
in ultrasonic bath with temperature regulation (Bandelin Elec-
tronic, Sonorex RK 100H, Germany) at 60 ◦ C for 30 min. Extract
was filtered, and plant material was extracted afterwards with two
portions
of the solvent by the same way. Extracts were filtered,
combined, and evaporated to dryness. The residues were dissolved
Soxhlet extraction2 g of dried E. arvense herb powder was placed to the filter paper,and extracted with applicable solvent (methanol or 80% aqueoussolution of methanol) for 48 h in Soxhlet apparatus. Extracts wereevaporated to dryness. The residues were dissolved in 25 mL of 80%aqueous solution of methanol. The whole procedure was repeatedthree times for each solvent. Ultrasound assisted extraction (USAE)2 g of dried E. arvense herb powder was extracted with 50 mL ofapplicable solvent (methanol or 80% aqueous solution of methanol)in ultrasonic bath with temperature regulation (Bandelin Elec-tronic, Sonorex RK 100H, Germany) at 60 ◦ C for 30 min. Extractwas filtered, and plant material was extracted afterwards with twoportionsof the solvent by the same way. Extracts were filtered,combined, and evaporated to dryness. The residues were dissolved
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