The vector
carry the left and right flanking sequences each with about 1 kb in
size from the tobacco plastid genome which are used for gene
insertion into trnR/trnN insertion site via homologous recombination.
The K2S coding regions were fused with 6(x) His-tag and a
factor Xa protease site for affinity chromatography purification
(Fig. 1). The vector was successfully transferred to tobacco plastomes
using the biolistic delivery procedure and homoplasmic
transplastomic plants were regenerated, as published previously
(Abdoli Nasab et al., 2013).