gravimetrically.
Preparation of protein reagent. Coomassie Brilliant Blue G-250 (100 mg)
was dissolved in 50 ml 95% ethanol. To this solution 100 ml 85% (w/v)
phosphoric acid was added. The resulting solution was diluted to a final
volume of I liter. Final concentrations in the reagent were 0.01% (w/v)
Coomassie Brilliant Blue G-250, 4.7% (w/v) ethanol, and 8.5% (w/v)
phosphoric acid.