The amplification procedure consisted of the following steps:5 min at 94◦C for initial denaturation, 35 cycles of denaturationfor 30 s at 94◦C, annealing for 30 s at 55◦C, extension for 30 s at 72◦C, and a final extension for 7 min at 72◦C. Next, 1.5% agarose gelelectrophoresis was performed to verify the amplification products.