Initially, once the extraction time was not pre-established, four extraction curves were constructed, as shown in Fig. 3, one for each experimental condition. The curves were constructed in terms of cumulative yield of dry extract (discounting the mass of citric acid added) as a function of S/F (mass of solvent used in the extraction/initial mass of raw material). Three different solvents, pure scCO2, ethanol and water both acidified with 0.3% citric acid, were used at the following operating conditions: 40 °C/300 bar, 40 °C/400 bar, 50 °C/300 bar, and 50 °C/400 bar. Overall, there was no significant difference between the total cumulative yields of the three steps, which ranged from 37% to 39%.