Histological Observation: Further observation based on
DPX. The slides then were left overnight before observed histological technique was done to confirmed the stage of
under an advanced microscope. ovarian development obtained in every individual shrimps
Data Analysis: The data taken by physical observation of stage I (immature), stage II (developing), stage III (ripe)
the external appearance of ovary was collected every and stage IV (spent). Figure 3 to 8 show the cross section
5 days at Day 5, Day 10, Day 15 and Day 20 based on the (5 μm) of the F. merguiensis ovary for different stage
characteristic described by [7]. Slides from histology at obtained from this study. 30 oocytes diameter from every
Day 20 were observed under advanced microscope shrimps was measured and data of mean oocytes diameter
(Nikon ECLIPSE 80i) and images were taken using (Table 3) was analyzed using one way ANOVA (analysis
NIS-Elements D 2.30 Software. Types of oocyte present in of variance).
(Table 2). Three different stages were observed, named