This work presents the first insight on the aflatoxin M 1 (AFM 1 ) occurrence in raw milk collected from
Macedonia, during the mycotoxin incidence in 2013. The total tested samples number was 3635,
collected from February 2013 to January 2014. They were analyzed for AFM 1 with ELISA as screening, and
HPLC e fluorescence detection as confirmatory method. The AFM 1 concentrations exceeded the
maximum permitted levels in 2.9% of the samples, and the highest detected concentration was 408.1 ng/
kg. The follow-up actions including feed sampling revealed aflatoxin B 1 contamination in 31.8% of the
samples tested, applying the HPLC-FD method. We estimated the carry-over rate between 0.22 and
3.47%. The experience obtained from the coordinated milk-feed aflatoxin survey could contribute to-
wards increasing awareness for establishing continuous monitoring systems.