2.6.2. Step 2
2.6.2.1. Determination of As3+. Six replicate of digested samples (5 ml) were taken in separating funnel, added 10 ml of 1 M HCl and placed in ultrasonic bath for 5 min. Added 10 ml of chloroform and placed in ultrasonic bath for 1 min (twicely), kept at room temperature to separate two phase. The chloroform fractions was poured to the second separating funnel, and As3+ was extracted with 1 M HCl and determined by ETAAS.