4. Discussion
Currently, RT-PCR is considered to be a convenient, useful, and powerful method for molecular diagnosis, to detect pathogens from clinical specimens. It has been used widely. In addition,RT-multiplex PCR assay provides simple, rapid, and cost-effective laboratory diagnosis. In this study, a rapid assay to detect simultaneously Aichi virus, HPeV, EVs, and HBoV in fecal specimens was developed.