Verticillium dahliae is a pathogen causing vascular wilts in a broad range of plant species. The production
of cell wall degrading enzymes (CWDE) is one of the pathogenicity factors contributing to this disease.
The expression of eight CWDE genes was estimated in 15 V. dahliae isolates with different hosts of origin
and aggressiveness levels. The tested isolates had similar levels of gene expression after12 h, 24 h, 48 h,
and 72 h in the presence of host plant root extracts. Nevertheless, correlation tests revealed a clear
association between the pathogenicity and gene expression of pectinase, cutinase, 1,4-b glucosidase, and
SNF protein kinase. In contrast, xylanase and endoglucanase G1 correlated negatively with pathogenicity.
Over time, V. dahliae produced CWDEs in a sequence, in which pectinases were produced earlier than
xylanase and endoglucanase G1. Correlation of gene expression results with the respective hosts of origin
of the tested V. dahliae isolates indicated that the latter may be producing their CWDEs according to the
cell wall composition of the hosts. Phylogenetic analysis of cDNA-AFLP profiles using V. dahliae mRNA
revealed a cohesive clade formed by the isolates from olive and potato, whereas those from sunflower
formed a genetically isolated clade. EST sequencing data revealed the exclusive expression of acetylxylan
esterase and ornithine decarboxylase in the case of sunflower isolates.
© 2015 E
Verticillium dahliae is a pathogen causing vascular wilts in a broad range of plant species. The productionof cell wall degrading enzymes (CWDE) is one of the pathogenicity factors contributing to this disease.The expression of eight CWDE genes was estimated in 15 V. dahliae isolates with different hosts of originand aggressiveness levels. The tested isolates had similar levels of gene expression after12 h, 24 h, 48 h,and 72 h in the presence of host plant root extracts. Nevertheless, correlation tests revealed a clearassociation between the pathogenicity and gene expression of pectinase, cutinase, 1,4-b glucosidase, andSNF protein kinase. In contrast, xylanase and endoglucanase G1 correlated negatively with pathogenicity.Over time, V. dahliae produced CWDEs in a sequence, in which pectinases were produced earlier thanxylanase and endoglucanase G1. Correlation of gene expression results with the respective hosts of originof the tested V. dahliae isolates indicated that the latter may be producing their CWDEs according to thecell wall composition of the hosts. Phylogenetic analysis of cDNA-AFLP profiles using V. dahliae mRNArevealed a cohesive clade formed by the isolates from olive and potato, whereas those from sunflowerformed a genetically isolated clade. EST sequencing data revealed the exclusive expression of acetylxylanesterase and ornithine decarboxylase in the case of sunflower isolates.© 2015 E
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