The reaction mixture contained 0.05 mL of the supernatant,
3 mL of 0.02 mol L−1 L-phenylalanine and borate buffer
(0.05 mol L−1, pH 8.7) in a total volume of 3.05mL, and it was incubated
at 30 °C for 30 min. The reaction was stopped by the addition of 0.5 mL
HCl (6 mol L−1). The absorbance of solution was measured at 290 nm
using a spectrophotometer. The PAL activity was expressed as
A h−1 g−1 FW.