Although it was found in this study that the effects of the reaction temperature, oil-to-methanol ratio, and reaction time on the efficiency of the transesterification of microalgal oil were similar to those reported in related studies (Dang et al., 2013; Zu et al., 2010), the efficiency obtained in this work was higher than that in the related literature when the reaction was conducted at a low temperature (e.g., 35 C).