Volunteer’s eligibility for study participation was determined
on the first visit through a thorough history taking and a
detailed clinical examination. Pregnancy test was done only when
appropriate. At the start of the study volunteers were subjected to
an assessment of wrinkles using Glogau Photo-aging Classification
defined as follows; type I ‘‘no wrinkles,’’ type II ‘‘winkles in motion,’’
type III ‘‘wrinkles at rest’’ and type IV ‘‘only wrinkles’’ as described
in Table 2.