Abstract
The purpose of this study is to develop a novel scaffold,
derived from fish scales, as an alternative functional
material with sufficient mechanical strength for corneal
regenerative applications. Fish scales, which are usually
considered as marine wastes, were acellularized, decalcified
and fabricated into collagen scaffolds. The microstructure
of the acellularized scaffold was imaged by scanning
electron microscopy (SEM). The acellularization and
decalcification treatments did not affect the naturally 3-dimentional, highly centrally-oriented micropatterned
structure of the material. To assess the cytocompatibility
of the scaffold with corneal cells, rabbit corneal cells were
cultured on the scaffold and examined under SEM and
confocal microscopy at different time periods. Rapid cell
proliferation and migration on the scaffold were observed
under SEM and confocal microscopy.
AbstractThe purpose of this study is to develop a novel scaffold,derived from fish scales, as an alternative functionalmaterial with sufficient mechanical strength for cornealregenerative applications. Fish scales, which are usuallyconsidered as marine wastes, were acellularized, decalcifiedand fabricated into collagen scaffolds. The microstructureof the acellularized scaffold was imaged by scanningelectron microscopy (SEM). The acellularization anddecalcification treatments did not affect the naturally 3-dimentional, highly centrally-oriented micropatternedstructure of the material. To assess the cytocompatibilityof the scaffold with corneal cells, rabbit corneal cells werecultured on the scaffold and examined under SEM andconfocal microscopy at different time periods. Rapid cellproliferation and migration on the scaffold were observedunder SEM and confocal microscopy.
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