Although the longer pressurization times and the matrix (PBS) used in this study are for research purposes and are not comparable to the ones used in food industry, they are selected as a the first step to study the effect of HPP technology in inactivation of enterotoxic and non-enterotoxic S. aureus and evaluation of its impact on virulence factors. Further steps should include studies in foods prompt to be contaminated with S. aureus to check its HPP resis- tance behaviour and, when HPP conditions are established, apply those conditions in food industry.