Date palm fruits were purchased from a main market of Akure, Nigeria and transported to the Microbiological Laboratory of Federal University of Technology, Akure, Nigeria. The fruits were sorted and the apparently healthy fruits were washed, subjected to natural fermentation at 25±2°C for five days and samples were examined at each day of fermentation. Colonies were isolated and subjected to biochemical identification (Erdoğrul and Erbilir, 2006; Gueimonde et al., 2004). New set of apparently healthy ones were subjected to controlled fermentation using the single culture and mixed culture of the predominant cultures obtained from the fruits in a modified bioreactor at 25°C for 5 days (Adegbehingbe et al., 2014). Samples were aseptically withdrawn from the solution throughout the fermentation period and subjected to chemical, nutritional and microbiological analyses. Samples were also filtered and presented for sensory evaluation.