2.5.1. Aerobic plate count
Aerobic plate counts were determined by inoculating 0.1 ml of
the homogenate sample onto triplicate sterile plates of pre poured and dried Standard Method Agar. Then, plates were
incubated for 48 h at 35 C (ISO NF- 21149, 2006). Duplicates
of each dilution (1 ml) of neutralized and non neutralized samples were pour-plated using Standard Method Agar (Oxoid,
Basingstoke, Hampshire, England) and incubated at 30 C
for 48 h. Plates containing 25–250 colonies were counted and
the average number of CFU/g was calculated.