Proteins linked
to reproduction (vitellogenins) appeared in the hemolymph 10 days
after adult emergence and indicated the end of the pre-mating stage.
After mating, total protein concentration in the hemolymph was lower
compared to virgin females; vitellogenin levels were similar during
most of the observation period. Oocyte development and maturation
were gradual and age dependent. Ten-day-old females had chorionated
oocytes ready for fertilization. Mating did not stimulate oocyte
development in N. viridula, but the lack of mating activity appeared
to have stimulated oocyte resorption in 17-day-old females.