Twenty previously reported STS primer pairs developed from RAPD (12, 13, 17) were tested in this study.
To identify the amplicon sequences,the amplified bands were excised, purified, TA cloned, and sequenced.
In a homology search,each cloned sequence was compared against all sequences in the
nonredundant databases using the BLASTX and BLASTN programs (http://www.ncbi.nlm.nih.gov/BLAST and www.gramene.org).
How ever, only one of the six markers, SSIIa from Bao et al.
(3), was polymorphic among the 22 rice varieties and was actually used in this
study.
The sequences and banding patterns of markers previously reported are presented inTable 1andFigure 1, respectively.