4. Conclusions
In summary, this work demonstrated that nanopore array
coupled with in-direct FT-RIS can be successfully acted as a sensing
platform to detect the pore blockage effect. Without complicated
manufacturing process encountered in single nanopore
technology, the nanopore array can be simply obtained from
electrochemically etched silicon, whose pore diameter can be
manipulated by electrochemical current. The immuno-captured
bacteria can effectively block the channel of nanopore array, resulting
in the reduction of pore accessibility. With the assistance of
FT-RIS, the pore accessibility can be sensitively probed by proteins
with suitable size. Compared with conventional nanopore technology,
this approach does not need the translocation of target
analyte through a nanopore. Therefore, microorganism such as
bacteria and cell can be conveniently detected on this platform.
The pervasive sensing strategy established in this work may expand
the application of nanopore array technology to more diverse
field.