24 = 17.08, P < 0.0001) and lower culture volume(ESALQ1432: F1, 24=62.29, P<0.0001; GHA: F1, 24=13.31, P<0.0001) together significantly increased biomassaccumulation by both isolates. The lowest blastospore yieldsand biomass accumulations occurred in oxygen-limited growncultures (i.e., 175 rpm and 100 mL) (Table 1). Isolate ESALQ1432 was more responsive than GHA to oxygen availabilityshowing greater biomass accumulation when cultivated inoxygen-rich media. During early growth times (<24 h), theoxygen level was saturated (100 %). Thereafter, DO levelsdeclined as biomass increased after 24 h fermentation. AlthoughGHA cultures apparently consumed more glucosethan ESALQ1432 cultures (F1, 24=17.07, P=0.0004), it wasnoticed that glucose consumption was significantly greater forboth isolates grown with higher oxygen availability as in lowerculture volume (F1, 24=9.21, P=0.006) and at faster agitationfor GHA cultures only (F1, 24=8.86, P=0.007) (Table 1).The final pH in the culture broth was characterized as moderatelyacidic for both strains with a range of 4.5–5.1. Thehighest speed of agitation (350 rpm) combinedwith the lowestculture volume (50 mL) resulted in the least viscous culture ofB. bassiana compared with poorly aerated cultures. The fasterDO levels decreased the more viscous cultures became accompaniedby fewer blastospores and increased mycelialgrowth
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