It is well-known that water transformation into very small ice crystals preserves the food structure during freezing process. Crystallization is a phase transition phenomena occurring during the freezing process which progresses through two steps: the formation of nuclei (namely nucleation) and subsequent growth of the nuclei to a specific crystal size, orientation and shape (Kiani and Sun, 2011). The nucleation, which strongly influences the morphology, size and distribution of ice crystals, is considered as a critical factor for the optimization of freezing industrial processes (Nakagawa et al., 2006; Petzold and Aguilera, 2009).