3.1.1. HPLC analysis of capsaicinoids
The contents of capsaicinoids were analyzed by HPLC to evaluate
the extracted effect. The HPLC separation conditions including
the ratio of mobile phase (methanol-water) and flow rate were
firstly investigated and the satisfactory chromatographic conditions
were summarized in Section 2.5.1. The HPLC chromatograms
of standard compound (capsaicin) and the crude extract were
shown in Fig. 2, respectively.